Mycobiotic Agar is a medium used for the selective cultivation of fungal pathogens from diverse clinical samples and other materials contaminated with a mixed associated flora. This medium is basically a mycological agar to which chloramphenicol and cycloheximide have been added. Chloramphenicol is an antibiotic that helps isolate pathogenic fungi from highly contaminated samples, since it inhibits most contaminating bacteria. It is an antibiotic recommended for use in media due to its thermal stability and its broad bacterial spectrum. Cycloheximide is an antibiotic that inhibits saprophytic fungi but allows the growth of pathogenic fungi.

Soy peptone provides nitrogen, vitamins, minerals, and amino acids essential for growth. Dextrose is the fermentable carbohydrate which is a carbon and energy source. Bacteriological agar is the solidifying agent. Dermatophytes and other numerous groups of pathogenic fungi grow quickly in the Mycobiotic Agar as it inhibits most of the bacterial and fungal saprophytes or commensal contaminants.