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pColiExpress™ IV LIC Cloning & Expression Kit, for a highly efficient & versatile protein expression (lic technology based) in e. coli from pcr fragment cloned

pColiExpress™ IV LIC Cloning &amp, Expression Kit is a highly efficient, versatile and fast system of DNA cloning vectors for protein expression in E. coli. All family systems are based in directional ligase-independent-cloning (LIC) technology, a rapid procedure that provides high-cloning efficiencies and the fast production of a large quantity of any desired protein. It contains all elements required for the expression of large quantities of any desired protein by the T7 RNA Polymerase Inducible System, and a His6 tag at COOH end of the protein that allows the purification. It contains at the NH2 end the Cmyc tag followed by a 3C from human Rhinovirus (HRV) Protease cleavage site, too. The vector has also an f1 origin of replication, an Ampicillin resistance cassette, and the pBR322 origin of replication.

Main Features:

Ready-to-use vectors. Highly efficient cloning system. Special design that allows the cell to house larger numbers of copies than other plasmids with ori pBR322. Linearized vector: ready for ligation with your PCR amplified with the recommended primers. Minimum background: lower than 1%. Time-saving protocol: avoids any step required after PCR. Higher protein expression than other suppliers. Cost avoidance: avoids the use of expensive phosphorylated primers. Versatility: cloning of PCR fragments amplified with any type of DNA Polymerase. Risk-free: product covered by Canvax Quality 100% Guarantee.

Includes: Includes for 20 rxn: - 20 µL pColiExpress™ linearized vector (50 ng/µL) - 50 µL LIC Enzyme Buffer (10x) - 40 µL LIC Enzyme (10 U/µL) - 10 µL Insert Control DNA (30 ng/µL) - 5 µL pColiExpress™ Control - Uncut vector (50 ng/µL)

Applications: Cloning of PCR fragments for subsequent expression of proteins in E. coli. Cloning of PCR fragments with Glue Enzyme. Expression of proteins under the control of the T7 promoter. Protein expression in BL21 (DE3) or BL21 (DE3) (pLys).

pColiExpress™ IV LIC Cloning & Expression Kit, for a highly efficient & versatile protein expression (lic technology based) in e. coli from pcr fragment cloned

pColiExpress™ IV LIC Cloning &amp, Expression Kit is a highly efficient, versatile and fast system of DNA cloning vectors for protein expression in E. coli. All family systems are based in directional ligase-independent-cloning (LIC) technology, a rapid procedure that provides high-cloning efficiencies and the fast production of a large quantity of any desired protein. It contains all elements required for the expression of large quantities of any desired protein by the T7 RNA Polymerase Inducible System, and a His6 tag at COOH end of the protein that allows the purification. It contains at the NH2 end the Cmyc tag followed by a 3C from human Rhinovirus (HRV) Protease cleavage site, too. The vector has also an f1 origin of replication, an Ampicillin resistance cassette, and the pBR322 origin of replication.

Main Features:

Ready-to-use vectors. Highly efficient cloning system. Special design that allows the cell to house larger numbers of copies than other plasmids with ori pBR322. Linearized vector: ready for ligation with your PCR amplified with the recommended primers. Minimum background: lower than 1%. Time-saving protocol: avoids any step required after PCR. Higher protein expression than other suppliers. Cost avoidance: avoids the use of expensive phosphorylated primers. Versatility: cloning of PCR fragments amplified with any type of DNA Polymerase. Risk-free: product covered by Canvax Quality 100% Guarantee.

Includes: Includes for 20 rxn: - 20 µL pColiExpress™ linearized vector (50 ng/µL) - 50 µL LIC Enzyme Buffer (10x) - 40 µL LIC Enzyme (10 U/µL) - 10 µL Insert Control DNA (30 ng/µL) - 5 µL pColiExpress™ Control - Uncut vector (50 ng/µL)

Applications: Cloning of PCR fragments for subsequent expression of proteins in E. coli. Cloning of PCR fragments with Glue Enzyme. Expression of proteins under the control of the T7 promoter. Protein expression in BL21 (DE3) or BL21 (DE3) (pLys).

pColiExpress™ IV Cloning & Expression Kit, for a highly efficient, fast & versatile protein expression (t4 dna ligase based) in e. coli from pcr fragment cloned

pColiExpress™ IV Cloning &amp, Expression Kit is a DNA Cloning System for Protein Expression in E.coli. pColiExpress™ technology allows efficiently cloning DNA fragments and the quick production of a large quantity of any desired Protein. pColiExpress™ IV contains all elements required for expression of large quantity of any desired protein by T7 RNA Polymerase Inducible System and a His6 tag at COOH end that allow the protein purification. It contains at NH2 end cmyc tag followed by PreScission Protease cleavage site, too. The vector has also an f1 origin of replication, an ampicillin resistance cassette, and a pBR322 origin of replication.

Main Features:

Ready-to-use vectors. Highly efficient cloning system. Special design that allows the cell to house larger numbers of copies than other plasmids with ori pBR322. Linearized vector: ready for ligation with your PCR amplified with the recommended primers. Minimum background: lower than 1%. Time-saving protocol: avoids any step required after PCR. Higher protein expression than other suppliers. Cost avoidance: avoids the use of expensive phosphorylated primers. Versatility: cloning of PCR fragments amplified with any type of DNA Polymerase. Risk-free: product covered by Canvax Quality 100% Guarantee.

Includes: Includes for 20 rxn: - 20 µL pColiExpress™ linearized vector (50 ng/µL) - 200 µL T4 DNA Ligase Buffer (5x) - 20 µL T4 DNA Ligase (5 U/µL) - 10 µL Control Insert DNA (30 ng/µL) - 5 µL pColiExpress™ Control (50 ng/µL) Plus Version Includes for 20 rxn - 20 µL pColiExpress™ linearized vector (50 ng/µL) - 200 µL T4 DNA Ligase Buffer (5x) - 20 µL T4 DNA Ligase (5 U/µL) - 10 µL Control Insert DNA (30 ng/µL) - 5 µL pColiExpress™ Control (50 ng/µL) - 20 µL EcoRV (10 U/µL) - 20 µL Hind III (10 U/µL) - 1.5 mL Buffer C (10x)

Applications: Cloning of PCR fragments for subsequent expression of proteins in E. coli. Expression of Proteins under the control of the T7 promoter. Protein expression in BL21 (DE3) or BL21 (DE3) (pLys).

pColiExpress™ IV Cloning & Expression Kit, for a highly efficient, fast & versatile protein expression (t4 dna ligase based) in e. coli from pcr fragment cloned

pColiExpress™ IV Cloning &amp, Expression Kit is a DNA Cloning System for Protein Expression in E.coli. pColiExpress™ technology allows efficiently cloning DNA fragments and the quick production of a large quantity of any desired Protein. pColiExpress™ IV contains all elements required for expression of large quantity of any desired protein by T7 RNA Polymerase Inducible System and a His6 tag at COOH end that allow the protein purification. It contains at NH2 end cmyc tag followed by PreScission Protease cleavage site, too. The vector has also an f1 origin of replication, an ampicillin resistance cassette, and a pBR322 origin of replication.

Main Features:

Ready-to-use vectors. Highly efficient cloning system. Special design that allows the cell to house larger numbers of copies than other plasmids with ori pBR322. Linearized vector: ready for ligation with your PCR amplified with the recommended primers. Minimum background: lower than 1%. Time-saving protocol: avoids any step required after PCR. Higher protein expression than other suppliers. Cost avoidance: avoids the use of expensive phosphorylated primers. Versatility: cloning of PCR fragments amplified with any type of DNA Polymerase. Risk-free: product covered by Canvax Quality 100% Guarantee.

Includes: Includes for 20 rxn: - 20 µL pColiExpress™ linearized vector (50 ng/µL) - 200 µL T4 DNA Ligase Buffer (5x) - 20 µL T4 DNA Ligase (5 U/µL) - 10 µL Control Insert DNA (30 ng/µL) - 5 µL pColiExpress™ Control (50 ng/µL) Plus Version Includes for 20 rxn - 20 µL pColiExpress™ linearized vector (50 ng/µL) - 200 µL T4 DNA Ligase Buffer (5x) - 20 µL T4 DNA Ligase (5 U/µL) - 10 µL Control Insert DNA (30 ng/µL) - 5 µL pColiExpress™ Control (50 ng/µL) - 20 µL EcoRV (10 U/µL) - 20 µL Hind III (10 U/µL) - 1.5 mL Buffer C (10x)

Applications: Cloning of PCR fragments for subsequent expression of proteins in E. coli. Expression of Proteins under the control of the T7 promoter. Protein expression in BL21 (DE3) or BL21 (DE3) (pLys).

pSpark® I DNA Cloning Kit, for highly efficient, accurate & robust general cloning from pcr high fidelity fragments, without toxic genes use

pSpark® I is a highly efficient, accurate and easy-to-use DNA cloning System based on a novel breakthrough technology to generate blunt vectors with a high blunt cloning efficiency. The vector is prepared by digestion of pSpark® at the EcoRV site before treating both ends to prevent vector self-ligation. The end treatment is supported by a proprietary know-how that guarantees a higher cloning efficiency than with simply a desphorylated vector.

Main Features:

Unprecedented high cloning efficiency: more than 2,500 positive colonies expected under optimal conditions. Easy-to-use: eliminate recombinant screening due to its minumum background (lower than 1%), avoiding "suicide" strategies from toxic genes. Time-saving protocol: no hidden steps such as phosphorylation, just ligation after PCR and transformation. High stability: eliminates cloning bias or pitfalls. Powerful: clone from less than 1 ng/kb, obtain 5-fold more positive colonies using 10x less DNA insert. Compatible with blue/white screening. Great versatility: compatible with any protocol, proofreading polymerase, competent cells, ligation time or primers. Sensitive: clone from 50 bp insert to up to 14 kb with just 5 ng per kb of insert. Eliminates positive selection vector. High cost-saving: reduces your cloning costs as no expensive phosphorylated primers are needed. Robust for every DNA size: just 6.7 ng per kb of insert needed for optimal ligation. Risk-free: product covered by Canvax Quality 100% Guarantee.

Includes: Includes for 20 rxn: - 20 µL pSpark® I (20 ng/µL) - 20 µL T4 DNA Ligase (5U/Weiss) - 200 µL T4 DNA Ligase Buffer (5x) - 150 µL PEG 6000 (10x) - 5 µL Insert Control 1 kb (20 ng/µL)

Applications: General cloning. Cloning of High Fidelity PCR amplified products. Production of ssDNA. Blue/white screening for recombinants. In vitro transcription from T7/SP6 dual-opposed promoters.

pSpark® I DNA Cloning Kit, for highly efficient, accurate & robust general cloning from pcr high fidelity fragments, without toxic genes use

pSpark® I is a highly efficient, accurate and easy-to-use DNA cloning System based on a novel breakthrough technology to generate blunt vectors with a high blunt cloning efficiency. The vector is prepared by digestion of pSpark® at the EcoRV site before treating both ends to prevent vector self-ligation. The end treatment is supported by a proprietary know-how that guarantees a higher cloning efficiency than with simply a desphorylated vector.

Main Features:

Unprecedented high cloning efficiency: more than 2,500 positive colonies expected under optimal conditions. Easy-to-use: eliminate recombinant screening due to its minumum background (lower than 1%), avoiding "suicide" strategies from toxic genes. Time-saving protocol: no hidden steps such as phosphorylation, just ligation after PCR and transformation. High stability: eliminates cloning bias or pitfalls. Powerful: clone from less than 1 ng/kb, obtain 5-fold more positive colonies using 10x less DNA insert. Compatible with blue/white screening. Great versatility: compatible with any protocol, proofreading polymerase, competent cells, ligation time or primers. Sensitive: clone from 50 bp insert to up to 14 kb with just 5 ng per kb of insert. Eliminates positive selection vector. High cost-saving: reduces your cloning costs as no expensive phosphorylated primers are needed. Robust for every DNA size: just 6.7 ng per kb of insert needed for optimal ligation. Risk-free: product covered by Canvax Quality 100% Guarantee.

Includes: Includes for 20 rxn: - 20 µL pSpark® I (20 ng/µL) - 20 µL T4 DNA Ligase (5U/Weiss) - 200 µL T4 DNA Ligase Buffer (5x) - 150 µL PEG 6000 (10x) - 5 µL Insert Control 1 kb (20 ng/µL)

Applications: General cloning. Cloning of High Fidelity PCR amplified products. Production of ssDNA. Blue/white screening for recombinants. In vitro transcription from T7/SP6 dual-opposed promoters.

pSpark® II DNA Cloning Kit, for highly efficient, accurate & easy general cloning with classical mcs, without the use of toxic genes

pSpark® II is a highly efficient, accurate and easy-to-use DNA Cloning system based on a breakthrough technology for cloning blunt ended DNA generated by PCR with a proofreading or high fidelity DNA polymerases. The vector is prepared by digestion of pSpark® II at the EcoRV site before treating both ends to prevent vector self-ligation. The end treatment is supported by a proprietary know-how that guarantees a higher cloning efficiency than with simply a desphorylated vector.

Main Features:

Unprecedented high cloning efficiency: more than 2,500 positive colonies expected under optimal conditions. Great efficiency: over hundreds positive colonies with few nanograms of insert. High stability: eliminates cloning bias or pitfalls. Time-saving protocol: no hidden steps such as phosphorylation, just ligation after PCR and transformation. Powerful: clone from less than 1 ng/kb to up to 14 kb

Includes: Includes for 20 rxn: - 20 µL pSpark® II (20 ng/µL) - 20 µL T4 DNA Ligase (5U/Weiss) - 200 µL T4 DNA Ligase Buffer (5x) - 150 µL PEG 6000 (10x) - 5 µL Insert Control 1 kb (20 ng/µL)

Applications: General cloning. Clone PCR fragments included in a low amount. Cloning of PCR products amplified with High Fidelity polymerases. Cloning of PCR fragments generated with a blend of polymerases. Production of ssDNA. Blue/white screening for recombinants. In vitro transcription from T7/SP6 dual-opposed promoters.

pSpark® II DNA Cloning Kit, for highly efficient, accurate & easy general cloning with classical mcs, without the use of toxic genes

pSpark® II is a highly efficient, accurate and easy-to-use DNA Cloning system based on a breakthrough technology for cloning blunt ended DNA generated by PCR with a proofreading or high fidelity DNA polymerases. The vector is prepared by digestion of pSpark® II at the EcoRV site before treating both ends to prevent vector self-ligation. The end treatment is supported by a proprietary know-how that guarantees a higher cloning efficiency than with simply a desphorylated vector.

Main Features:

Unprecedented high cloning efficiency: more than 2,500 positive colonies expected under optimal conditions. Great efficiency: over hundreds positive colonies with few nanograms of insert. High stability: eliminates cloning bias or pitfalls. Time-saving protocol: no hidden steps such as phosphorylation, just ligation after PCR and transformation. Powerful: clone from less than 1 ng/kb to up to 14 kb

Includes: Includes for 20 rxn: - 20 µL pSpark® II (20 ng/µL) - 20 µL T4 DNA Ligase (5U/Weiss) - 200 µL T4 DNA Ligase Buffer (5x) - 150 µL PEG 6000 (10x) - 5 µL Insert Control 1 kb (20 ng/µL)

Applications: General cloning. Clone PCR fragments included in a low amount. Cloning of PCR products amplified with High Fidelity polymerases. Cloning of PCR fragments generated with a blend of polymerases. Production of ssDNA. Blue/white screening for recombinants. In vitro transcription from T7/SP6 dual-opposed promoters.

pSpark® III DNA Cloning Kit, for highly efficient, accurate & easy cloning with ampicillin and kanamycin resistance

pSpark® III is a highly efficient, accurate and easy-to-use DNA cloning system that combines Ampicillin and Kanamycin resistance. Ideal for cloning PCR products amplified from any plasmid vector without the need to gel-purify bands to eliminate the background due to the template vector used for PCR.

Main Features:

Unprecedented high cloning efficiency: more than 2,500 positive colonies expected under optimal conditions. Easy-to-use: eliminate recombinant screening due to its minumum background (lower than 1%), avoiding "suicide" strategies from toxic genes. Time-saving protocol: no hidden steps such as phosphorylation, just ligation after PCR and transformation. High stability: eliminates cloning bias or pitfalls. Powerful: clone from less than 1 ng/kb, obtain 5-fold more positive colonies using 10x less DNA insert. Compatible with blue/white screening. Great versatility: compatible with any protocol, proofreading polymerase, competent cells, ligation time or primers. Sensitive: clone from 50 bp insert to up to 14 kb with just 5 ng per kb of insert. Eliminates positive selection vector. High cost-saving: reduces your cloning costs as no expensive phosphorylated primers are needed. Robust for every DNA size: just 6.7 ng per kb of insert needed for optimal ligation. Risk-free: product covered by Canvax Quality 100% Guarantee.

Includes: Includes for 20 rxn: - 20 µL pSpark® III (20 ng/µL) - 20 µL T4 DNA Ligase (5U/Weiss) - 200 µL T4 DNA Ligase Buffer (5x) - 150 µL PEG 6000 (10x) - 5 µL Insert Control 1 kb (20 ng/µL)

Applications: Cloning directly from PCR using plasmid cloned genes as template. Unpurified PCR cloning. Cloning of high fidelity PCR amplified products. Production of ssDNA. Blue/white screening for recombinants. In vitro transcription from T7/SP6 dual-opposed promoters.

pSpark® III DNA Cloning Kit, for highly efficient, accurate & easy cloning with ampicillin and kanamycin resistance

pSpark® III is a highly efficient, accurate and easy-to-use DNA cloning system that combines Ampicillin and Kanamycin resistance. Ideal for cloning PCR products amplified from any plasmid vector without the need to gel-purify bands to eliminate the background due to the template vector used for PCR.

Main Features:

Unprecedented high cloning efficiency: more than 2,500 positive colonies expected under optimal conditions. Easy-to-use: eliminate recombinant screening due to its minumum background (lower than 1%), avoiding "suicide" strategies from toxic genes. Time-saving protocol: no hidden steps such as phosphorylation, just ligation after PCR and transformation. High stability: eliminates cloning bias or pitfalls. Powerful: clone from less than 1 ng/kb, obtain 5-fold more positive colonies using 10x less DNA insert. Compatible with blue/white screening. Great versatility: compatible with any protocol, proofreading polymerase, competent cells, ligation time or primers. Sensitive: clone from 50 bp insert to up to 14 kb with just 5 ng per kb of insert. Eliminates positive selection vector. High cost-saving: reduces your cloning costs as no expensive phosphorylated primers are needed. Robust for every DNA size: just 6.7 ng per kb of insert needed for optimal ligation. Risk-free: product covered by Canvax Quality 100% Guarantee.

Includes: Includes for 20 rxn: - 20 µL pSpark® III (20 ng/µL) - 20 µL T4 DNA Ligase (5U/Weiss) - 200 µL T4 DNA Ligase Buffer (5x) - 150 µL PEG 6000 (10x) - 5 µL Insert Control 1 kb (20 ng/µL)

Applications: Cloning directly from PCR using plasmid cloned genes as template. Unpurified PCR cloning. Cloning of high fidelity PCR amplified products. Production of ssDNA. Blue/white screening for recombinants. In vitro transcription from T7/SP6 dual-opposed promoters.

pSpark® IV DNA Cloning Kit, for highly efficient, stable & powerful cloning under transcription-free conditions

pSpark® IV is a highly efficient, accurate and easy-to-use DNA cloning system that exploit its very low background feature for the expression of toxic genes under transcription-free conditions. In this vector, the lac promoter has been eliminated and therefore blue/white screening is not allowed (alpha-peptide coding region remains and you can find blue colony). The vector is ideal for cloning genes that produce toxic polypeptides by transcription/ translation.

Main Features:

Unprecedented high cloning efficiency: more than 2,500 positive colonies expected under optimal conditions. Transcription-free. Easy-to-use: eliminate screening of recombinants due to its minumum background (lower than 1%). High stability: eliminates cloning bias or pitfalls. Time-saving protocol: avoids any step required after PCR, just 19 minutes from PCR to plating. Powerful: obtain 5-fold more positive colonies using 10-fold less DNA insert. Great versatility: compatible with any protocol, proofreading Polymerase, Competent Cells, ligation time or primers. Sensitive: clone from 50 bp insert to up to 14 kb with just 5 ng per kb of insert. Cost avoidance: removes expensive primer phosphorylation use. Eliminates positive selection vector. Risk-free: product covered by Canvax Quality 100% Guarantee.

Includes: Includes for 20 rxn: - 20 µL pSpark® IV (20 ng/µL) - 20 µL T4 DNA Ligase (5U/Weiss) - 200 µL T4 DNA Ligase Buffer (5x) - 150 µL PEG 6000 (10x) - 5 µL Insert Control 1 kb (20 ng/µL)

Applications: Cloning of high fidelity PCR amplified products. Production of ssDNA. In vitro transcription from T7/SP6 dual-opposed promoters. Cloning of toxic genes.

pSpark® IV DNA Cloning Kit, for highly efficient, stable & powerful cloning under transcription-free conditions

pSpark® IV is a highly efficient, accurate and easy-to-use DNA cloning system that exploit its very low background feature for the expression of toxic genes under transcription-free conditions. In this vector, the lac promoter has been eliminated and therefore blue/white screening is not allowed (alpha-peptide coding region remains and you can find blue colony). The vector is ideal for cloning genes that produce toxic polypeptides by transcription/ translation.

Main Features:

Unprecedented high cloning efficiency: more than 2,500 positive colonies expected under optimal conditions. Transcription-free. Easy-to-use: eliminate screening of recombinants due to its minumum background (lower than 1%). High stability: eliminates cloning bias or pitfalls. Time-saving protocol: avoids any step required after PCR, just 19 minutes from PCR to plating. Powerful: obtain 5-fold more positive colonies using 10-fold less DNA insert. Great versatility: compatible with any protocol, proofreading Polymerase, Competent Cells, ligation time or primers. Sensitive: clone from 50 bp insert to up to 14 kb with just 5 ng per kb of insert. Cost avoidance: removes expensive primer phosphorylation use. Eliminates positive selection vector. Risk-free: product covered by Canvax Quality 100% Guarantee.

Includes: Includes for 20 rxn: - 20 µL pSpark® IV (20 ng/µL) - 20 µL T4 DNA Ligase (5U/Weiss) - 200 µL T4 DNA Ligase Buffer (5x) - 150 µL PEG 6000 (10x) - 5 µL Insert Control 1 kb (20 ng/µL)

Applications: Cloning of high fidelity PCR amplified products. Production of ssDNA. In vitro transcription from T7/SP6 dual-opposed promoters. Cloning of toxic genes.