Canvax SEAP Reporter Gene Assay Kit CA040

SEAP Reporter Gene Assay Kit, for a really easy, sensitive & fast assay, luminescence based, measurement the levels of seap in the culture medium

Secreted Alkaline Phosphatase (SEAP) Reporter Gene is a really easy, sensitive and fast assay that utilizes enzyme activity of alkaline phosphatase to dephosphorylate the chemiluminescent alkaline phosphatase substrate CSPD into an unstable dioxetane anion which decomposes and emits light. SEAP encodes a truncated form of the placental enzyme that lacks the membrane anchoring domain, thereby allowing the protein to secrete efficiently from transfected cells. Changes in levels of SEAP activity detected in the culture medium are directly proportional to changes in intracellular concentrations of SEAP mRNA and protein.

Main Features:

Convenient: a single set of cells is used for both the SEAP assay and another purpose. Time-saving protocol: results in 55 minutes due the elimination of cell lysate preparation. Cost-effective: allows performance directly in a microtiter plate. Sensitive: assayed even in low cell concentrations. Secreted from transfected cells into the culture medium. Risk-free: product covered by Canvax Quality 100% Guarantee.

Includes: Includes for 288 assays: - 3 x 96 W Solid Plate (white) - 3 units of lid - 50 µL Alkaline Phosphatase Standard - 15 mL SEAP Substrate (Luminescence)

Applications: Measurement the levels of SEAP in the culture medium of transfected cells.