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Canvax FDG - Galactosidase Assay Kit (Fluorimetric) CA083
PART NO: CX-CA083
FDG - Galactosidase Assay Kit (Fluorimetric), efficient, easy & highly sensitive tool, fluorimetric based, to quickly measure levels of active ß-galactosidase
FDG ß-Galactosidase Assay Kit is an efficient, easy and highly sensitive tool to quickly measure the levels of active ß-galactosidase expressed in cells transfected with plasmids expressing Lac Z. Lac Z is often used reporter gene in experiments transfection because the ß-galactosidase is very resistant to proteolytic degradation and its activity is easily measured. ß-galactosidase performs the hydrolysis of fluorescein di-ß-D galactopyranoside (FDG) into a fluorescein that can be detected at excitation/emission = 490/525 nm. The concentration of ß-galactosidase is proportional to fluorescence produced.measure levels of active ß-galactosidase expressed in cells transfected with plasmids expressing Lac Z.
Main Features:
Fast and convenient. Easy-to-use method to quantify the enzyme expression in transfected cells. Sensitive: measure ß-galactosidase at femtogram level. Risk-free: product covered by Canvax Quality 100% Guarantee.
Includes: Includes for 500 assays: - 55 mL Reaction Buffer - 25 mL Lysis Buffer - 25 mL Stop solution - 20 µL ß-galactosidase enzyme (0.1 mg/mL) - 500 µL ß-Mercaptoethanol - 125 µL FDG solution (10 mM)
Applications: Measurement of ß-Galactosidase activity in the lysates of transfected cell.
FDG ß-Galactosidase Assay Kit is an efficient, easy and highly sensitive tool to quickly measure the levels of active ß-galactosidase expressed in cells transfected with plasmids expressing Lac Z. Lac Z is often used reporter gene in experiments transfection because the ß-galactosidase is very resistant to proteolytic degradation and its activity is easily measured. ß-galactosidase performs the hydrolysis of fluorescein di-ß-D galactopyranoside (FDG) into a fluorescein that can be detected at excitation/emission = 490/525 nm. The concentration of ß-galactosidase is proportional to fluorescence produced.measure levels of active ß-galactosidase expressed in cells transfected with plasmids expressing Lac Z.
Main Features:
Fast and convenient. Easy-to-use method to quantify the enzyme expression in transfected cells. Sensitive: measure ß-galactosidase at femtogram level. Risk-free: product covered by Canvax Quality 100% Guarantee.
Includes: Includes for 500 assays: - 55 mL Reaction Buffer - 25 mL Lysis Buffer - 25 mL Stop solution - 20 µL ß-galactosidase enzyme (0.1 mg/mL) - 500 µL ß-Mercaptoethanol - 125 µL FDG solution (10 mM)
Applications: Measurement of ß-Galactosidase activity in the lysates of transfected cell.
| Format: 500 assays Shipping conditions: Gel Pack Storage Conditions: -20 ºC | |
| Manufacturer | Canvax |
| Manufacturer P/n | CA083 |